Automation and Analytical Techniques: Measurement of Folate in Fresh and Archival Serum Samples as p-Aminobenzoylglutamate Equivalents
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چکیده
RESULTS: The limit of quantification was 0.25 nmol/L, and the assay was linear in the range 0.25–96 nmol/L, which includes the 99.75 percentile for serum folate concentrations in healthy blood donors. Withinand between-day imprecision was 5%. We detected no residual folate in serum samples after sample preparation. Folate concentrations in fresh serum samples obtained with the pABG assay and with a microbiologic assay showed good agreement (r 0.96). In stored samples containing low folate concentrations due to folate degradation, the pABG assay yielded substantially higher folate concentrations than the microbiologic assay.
منابع مشابه
Measurement of folate in fresh and archival serum samples as p-aminobenzoylglutamate equivalents.
BACKGROUND The development of accurate and precise folate assays has been difficult, mainly because of folate instability. Large interassay and interlaboratory differences have been reported. We therefore developed a serum folate assay that measures folate and putative degradation products as p-aminobenzoylglutamate (pABG) equivalents following oxidation and acid hydrolysis. METHODS Serum was...
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تاریخ انتشار 2008